Bjørnar Synstad

Utdanning:

Dr. scient, Biologisk Institutt, Universitetet i Oslo. 1996.                    Fagfelt: mikrobiologi -fotosyntetiske bakterier                             

Cand. scient, Biologisk Institutt, UiO. 1988.                                                                              

Arbeidserfaring:

2013-  1. amanuensis ved Universitetet i Agder (UiA), Fakultet for teknologi og realfag. Institutt for naturvitenskapelige fag. Fast ansatt i 100% stilling fra 1.1 2016.

2008-2013. Diverse vikariater som 1. amanuensis og timelærer ved UiA. Fakultet for teknologi og realfag. Insitutt fornaturvitenskapelige fag. 

2006-2007. Vikariat som 1. amanuensis ved Høgskolen i Østfold (HiØ), avd. for ingeniørfag i Sarpsborg, seksjon kjemi. 

2001-2005. Forsker ved Institutt for kjemi, bioteknologi og matvitenskap, Norges landbrukshøgskole/UMB. Ansatt på et NFR-prosjekt (”Design and engineering of novel chitinases”).

1997-2000. Forsker ved Institutt for Bioteknologi fag, Norges landbrukshøgskole (NLH). Ansatt på et EU-prosjekt (”Protein design studier av kitinaser” ).

1995-1996. Forsker ved Institutt for farmakologi, mikrobiologi og næringsmiddelhygiene, seksjon for næringsmiddelhygiene, Norges veterinærhøgskole.

1989-1994. Universitetsstipendiat, Universitetet i Oslo.

Vitenskapelige publikasjoner:

Synstad B, Vaaje-Kolstad G, Cederkvist H, Saua SF, Horn SJ, Eijsink VGH, Sørlie M (2008) Expression and Characterization of endochitinase C fromSerratia marcescensBJL200 and its purification by a one-step general chitinase purification method. Biosci. Biotechnol. Biochem.73: 715-723.

Krokeide I-M., Synstad B., Gåseidnes S., Horn S. J., Eijsink VGH. and Sørlie M. (2007) Natural substrate assay for chitinases using high- performance liquid chromatography: A comparison with existing assays. Anal. Biochemistry. 363: 128-134.

Horn SJ, Sikorski P, Cederkvist JB, Vaaje-Kolstad G, Sørlie M, Synstad B, Vriend G, Vårum KM, Eijsink VGH (2006): Cost and benefits of processivity in enzymatic degradation of recalcitrant polysacchaides. Proc. Natl. Acad. Sci. USA, 103: 18089-18094.

Horn SJ, Sørbotten A, Synstad B, Sikorski P, Sørlie M, Vårum KM, Eijsink VGH (2006)  Endo/exo mechanism and processivity of family 18 chitinases produced by Serratia marcescens. FEBS journal. 273:491-503.

Horn SJ, Sørlie M, Norberg AL, Synstad B, Vaaje-Kolstad G, Vårum KM, Eijsink VGH (2006) Comparative studies of chitinases A, B, and C from Serratia marcescens.
Biocatalysis and Biotransformation. 24 (1/2): 39-53

Vaaje-Kolstad G, Horn SJ, Van Aalten DMF, Synstad B, Eijsink VGH (2005) The non-catalytic chitin-binding protein CBP21 from Serratia marcescensis essential for chitin degradation. J. Biol. Chem. 280: 28492-28497.

Houston DR, Synstad B, Eijsink VG, Stark MJ, Eggleston IM, van Aalten DM (2004) Structure-based exploration of cyclic dipeptide chitinase inhibitors. J. Med. Chem. 47: 5713-5720.

Vaaje-Kolstad G, Houston DR, Rao FV, Peter MG, Synstad B, van Aalten DM, Eijsink VG (2004) Structure of the D142N mutant of the family 18 chitinase ChiB from Serratia marcescens and its complex with allosamidin.Biochim. Biophys. Acta. 1696:103-11.

Synstad B, Gåseidnes S, Van Aalten DMF, Vriend G, Nielsen JE, Eijsink VGH (2004) Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase.  Eur. J. Biochem. 271:253-62.

Vaaje-Kolstad G, Vasella A, Peter MG, Netter C, Houston DR, Westereng B, Synstad B, Eijsink VG, van Aalten DM (2004)Interactions of a family 18 chitinase with the designed inhibitor HM508 and its degradation product, chitobiono-delta-lactone. J. Biol. Chem. 279: 3612-3619

Gåseidnes S, Synstad B, Jia X, Kjellesvik H, Vriend G, Eijsink VG (2003) Stabilization of a chitinase fromSerratia marcescensby Gly-->Ala and Xxx-->Pro mutations. Protein Eng.16: 841-846

Gåseidnes S, Synstad B, Nielsen JE, Eijsink VGH (2003) Rational engineering of the stability and the catalytic performance of enzymes.      J. Mol. Catalysis B: Enzymatic 21. 3-8

Thiele G, Rottmann A, Germer A, Kleinpeter E, Spindler K-D, Synstad B, Eijsink VGH, Peter MG (2002) Synthesis and conformational analysis of pseudosugar analogues of chitoriose. J. Carbohydrate Chemistry 21: 471-89

Houston DR, Eggleston I, Synstad B, Eijsink VGH, Van Aalten DM (2002) The cyclic dipeptide CI-4 [cyclo-(L-Arg-D-Pro)] inhibits family 18 chitinases by structural mimicry of a reaction intermediate. Biochem. J. 368: 23-27.

Houston DR, Shiomi K, Arai N, Omura S, Peter MG, Turberg A, Synstad B, Eijsink VG, van Aalten DM (2002) High-resolution structures of a chitinase complexed with natural product cyclopentapeptide inhibitors: mimicry of carbohydrate substrate. Proc Natl Acad Sci USA. 99: 9127-32. 

Dalhus B, Saarinen M, Sauer UH, Eklund P, Johansson K, Karlsson A, Ramaswamy S, Bjork A, Synstad B, Naterstad K, Sirevag R, Eklund H (2002) Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases. J Mol Biol. 318:707-21.

Tabudravu JN, Eijsink VG, Gooday GW, Jaspars M, Komander D, Legg M, Synstad B, van Aalten DM (2002) Psammaplin A, a chitinase inhibitor isolated from the Fijian marine sponge Aplysinella rhax. Bioorg. Med Chem. 10:1123-8.

Kolstad G, Synstad B, Eijsink VG, van Aalten DM (2001) Structure of the D140N mutant of chitinase B from Serratia marcescensat 1.45 Å resolution.  Acta Crystallogr D Biol Crystallogr. 58: 377-9.

van Aalten DMF, Komander D, Synstad B, Gaseidnes S, Peter MG, Eijsink VG (2001) Structural insights into the catalytic mechanism of a family 18 exo-chitinase. Proc. Natl. Acad. Sci.USA, 98: 8979-8984

van Aalten  DMF, Synstad  B, Brurberg  MB, Hough  E, Riise  BW, Eijsink  VGH, Wierenga  RK  (2000)  Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-Å resolution. Proc. Natl. Acad. Sci.USA, 97: 5842-5847.

Rottmann  A, Synstad  B, Eijsink  V, Peter  MG (1999) Synthesis of N-acetylaminyl  and diacetyl chitobiosyl amides of heterocyclic carboxylic acids as potential chitinase inhibitors. Eur. J. Organic Chemistry 1999: 2293-2297.

Brynestad  S, Synstad  B,Granum PE(1997) The Clostridium perfringens enterotoxin gene is on a tranposable element in type A human food poisoning strains. Microbiology 143: 2109-2115.

Synstad  B, Emmerhoff O, Sirevåg  R (1996) Malate dehydrogenase from the green gliding bacterium Chloroflexus aurantiacus is phylogenetically related to lactic dehydrogenase. Arch. Microbiol.  165: 346-353.

Oversiktsartikler

Eijsink VGH, Kolstad G, Gåseidnes S, Synstad B, Peter MG, Nielsen JE, Komander D,Houston D, van Aalten DMF (2004) Details in the reaction mechanism of chitinases. Enzyme Functionality. Design, Engineering, and Screening, edited by Allan Svendsen. Marcel Dekker, Inc. New York.Basel.

Eijsink VG, Bjork A, Gaseidnes S, Sirevag R, Synstad B, van den Burg B, Vriend G. (2004) Rational engineering of enzyme stability. J. Biotechnol. 113:105-120.

Eijsink VGH, Synstad B, Kolstad G, Gåseidnes S, Komander D, Houston D, Peter MG, van Aalten DM (2003) Structure and function of chitinolytic enzymes. Advan. Chitin Sci. 6: 71-77.

Brurberg MB, Synstad B, Sletner Klemsdal S, van Aalten DMF, Sundheim L, Eijsink VGH (2001) Chitinases from Serratia marcescens. Recent Res.Devel. Microbiol. 5: 187-204.  

Rottman  A., Synstad  B., Thiele  G., Schanzenbach D., Eijsink V.G.H. and Peter M.G. (2000)  Approaches towards the design of new chitinase inhibitors, Advan. Chitin Sci. 4: 553-557.

Letzel, M.C., Synstad, B., Eijsink, V.G.H., Peter-Katalinic, J. and Peter, M.G. (2000)  Libraries of chito-oligosaccharides of mixed acetylation patterns and their interactions with chitinases, Advan. Chitin Sci.4: 545-552.

Synstad B., Gåseidnes S., Vriend G., Nielsen J.-E. and Eijsink V.G.H. (2000) On the contribution of conserved acidic residues to catalytic activity of chitinase B from Serratia marcescens, Advan. Chitin Sci. 4: 524-529.